Mycetoma caused by Gordonia soli

Red arrow indicates tiny hypointense foci within hyperintense spherical lesions, known as the “dot-in-circle” sign

France JCB et al. – Mycetoma caused by Gordonia soli

João César Beenke França ^[1] , Bruno Hassunuma Carneiro ^[1] , Regielly Caroline Raimundo Cognialli ^[1] and Flávio de Queiroz-Telles ^[2]

[1]. Federal University of Paraná, Hospital de Clínicas, Curitiba, PR, Brazil.

[two]. Federal University of Paraná, Department of Public Health, Curitiba, PR, Brazil.

Corresponding author: Dr. Flávio de Queiroz-Telles. email: queiroz.telles@uol.com.br

Authors’ contribution

JCBF, BHC, RCRC and FQ-T: Conceptualization; JCBF, BHC and RCRC: writing—original draft preparation; JCBF, BHC, RCRC and FQ-T: writing—review and editing; JCBF, BHC, RCRC and FQ-T: investigation and data curation.

Conflict of Interest

The authors declare no conflict of interest.

Financial Support

No external funding was received for this study.

Orcid

João César Beenke França: https://orcid.org/0009-0004-9909-9887

Bruno Hassunuma Carneiro: https://orcid.org/0000-0002-6022-2778

Regielly Caroline Raimundo Cognialli: https://orcid.org/0000-0002-3895-1982

Flávio de Queiroz-Telles: https://orcid.org/0000-0001-7034-2418

Received 11 July 2023 – Accepted 23 August 2023

Abstract

Mycetoma is a neglected tropical disease caused by fungi (eumycetoma) or bacteria (actinomycetoma), with high morbidity. Gordonia spp. are gram-positive bacteria that have previously been reported to cause mycetoma. Here, we report a case of Gordonia soli (initially misidentified as Nocardia spp.) as the etiological agent of actinomycetoma in a 64-year-old patient. After a literature search in the Cochrane Library, LILACS, SciELO, MEDLINE, PubMed, and PubMed Central databases, we concluded that this is the first case report of mycetoma caused by Gordonia soli . The current case highlights the importance of microbiological diagnosis of mycetoma and the challenges in its management.

Keywords: Mycetoma. Gordonia soli. actinomycetoma.

Introduction

Mycetoma is a neglected tropical disease characterized by the implantation of chronic granulomatous infection that occurs following a traumatic inoculation of a particular microorganism into the subcutaneous tissue, usually due to thorns or splints ¹ . The most commonly involved anatomical regions are the legs and feet, although the shoulders and upper back may also be affected when carrying wood logs or lying down in direct contact with the soil in endemic regions ¹ . The lesion progresses over months or even years after the traumatic injury, usually evolving from painless enlarging nodules to purulent lesions with draining fistulas and elimination of grains of variable sizes. It can be caused by fungi (Eumycetoma) or bacteria (Actinomycetoma) ^2,3 .

Most mycetoma cases have been reported in Mexico, India, and Sudan ^1,3 . In Mexico and India, 97% and 58% of the cases, respectively, were actinomycetomas, whereas in Sudan, 73% were eumycetoma ³ . In Brazil, nearly 300 mycetoma cases have been reported, with a high prevalence of actinomycetoma (75%) ⁴ .

The presumptive diagnosis of the microbiological etiology of both actinomycetoma and eumycetoma can be made based on the morphological characteristics of direct grain microscopic examination ⁵ . The causative agent of the mycetoma was identified through isolation from biological specimens ⁵ . Genus and species identification, which was historically performed using biochemical tests, can be achieved by molecular methods such as16S rRNA or Internal Transcribed Spacer (ITS) gene sequencing or matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) ^5.6 . Actinomycetoma is most commonly caused by Nocardia brasiliensis, Streptomyces somaliensis and Actinomadura madurae ^1,7 .

Here, we present a case of mycetoma caused by Gordonia soli , initially identified as Nocardia spp . Additionally, a literature review was performed using the Cochrane Library, LILACS, SciELO, MEDLINE, PubMed, and PMC (PubMed Central) databases.

Case Report

A 64-year-old man with no comorbidities presented with painful nodular lesions on his left foot following a traumatic injury. On examination, there were enlarging nodules that progressed to purulent lesions with bloody discharge, draining fistulas, and elimination of grains. A clinical diagnosis of mycetoma was made and a biopsy was performed. The histopathological evaluation revealed granulomas and fibrosis. It was also notable for the presence of grains and filamentous structures compatible with an actinomycetoma ( Figure 1–A, B, C ). Multiple tissue cultures were negative, and the causative agent was presumed to be Nocardia spp . based on clinical and histopathological findings.

The patient was treated with trimethoprim-sulfamethoxazole (TMP-SMX) for eight years, without complete resolution of symptoms, as he had persistent painful nodular draining lesions ( Figure 2-A ). A new culture was obtained from the draining secretion, and Gordonia soli was recovered by culture and identified using 16S rRNA gene sequencing ⁸ . He was treated with three courses of a 3-week regimen of intravenous linezolid (600 mg every 12 h) plus meropenem (1 g every 8 h) administered at intervals of 24 weeks, and oral TMP-SMX (8 mg/kg/day of the TMP component) was maintained between and after the courses. He experienced significant improvement in pain, edema, and resolution of the purulent discharge.

Following adequate symptom resolution, maintenance therapy with TMP-SMX was discontinued. Four years later, the patient experienced pain recurrence, edema, purulent discharge, and elimination of grains through the fistulas ( Figure 2-B ). A new specimen was obtained from the secretion, and the grains were observed using direct microscopy ( Figure 1-D ). The causative organism was cultured ( Figure 1–E ) and the genus Gordonia identified by MALDI-TOF MS (score 1.62) (Bruker MALDI Biotyper), which was confirmed as Gordonia soli by 16S rRNA gene sequencing, indicating persistence of infection. Magnetic resonance imaging (MRI) of the foot revealed signs suggestive of inflammation in the subcutaneous tissue; midtarsal and tarsometatarsal joints; and the calcaneum, midfoot, and metatarsal bones, suggesting osteomyelitis. The “dot-in-circle” sign was present, which is suggestive of mycetoma ( Figure 2-C ). The patient was treated with another two courses of intravenous linezolid plus meropenem, with significant improvement in symptoms, and maintenance therapy was indicated with oral TMP-SMX plus ciprofloxacin (500 mg every 8 h).

Discussion

In 1971, Gordona was identified as a new genus of bacteria from soil and sputum samples using biochemical tests. Initially, it had been classified under the genus Rhodococcus due to biochemical similarities. Gordona was renamed to Gordonia to honor the microbiologist Ruth E. Gordon in 1997 ⁹ .

Gordonia is a gram-positive, aerobic, partially acid-fast, fastidious bacterium that requires the use of enrichment media for isolation, and its phenotypic similarities to Rhodococcus and Nocardia make traditional biochemical identification a challenge ⁹ . The genus Gordonia comprises 39 different species ¹⁰ . Similar to other actinomycetes, its slow growth poses a difficulty in microbiological diagnosis, as conventional cultures are typically incubated for only 72 h. There are limitations to the identification of Gordonia by MALDI-TOF; therefore, a lower cutoff for identification at the genus (>1.5) and species levels (>1.7) was proposed ¹⁰ . Precise identification of genera and species is achieved by molecular methods such as 16S rRNA gene sequencing, which are only available in reference laboratories ^6,8,10-11 .

Members of the genus Gordonia are present in the soil and can be transferred through aerosols from environmental sources to the respiratory tract, thereby causing pulmonary infections, mainly in immunocompromised hosts. They can also cause bacteremia and spread to the central nervous system, cardiac tissues, and joints. G. sputi , G. bronchialis, and G. terrae are the most frequently identified species in clinical samples ⁹ .

Actinomycetoma is usually treated with long-term anti-infective medications. Recurrence is common and some patients require combined surgical management to achieve clinical success ^1,7 . Susceptibility testing for Gordonia was performed using broth microdilutions. Based on the reported isolates, it was most susceptible to imipenem, ciprofloxacin, amikacin, linezolid, gatifloxacin, gentamicin and vancomycin ⁹ . In the present case, susceptibility to TMP-SMX was demonstrated in vitro and surgical therapy was considered. However, this was not performed after a shared decision-making approach with the patient, as it was predicted to be mutilating.

Three cases of actinomycetoma caused by Gordonia have been reported: two caused by Gordonia terrae and one by Gordonia weatfalia ^11,12 . To the best of our knowledge, this is the first reported case of actinomycetoma caused by Gordonia soli.

Acknowledgments

We thank the team of the Infectious Diseases Department and Laboratory of Hospital de Clínicas from Federal University of Paraná.

References

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FIGURE 1: Microbiological and histopathological findings; (A) Grain in hematoxylin and eosin stain; (B) Grain in periodic Acid-Schiff stain; (C) Grain in Fite-Faraco stain; (D) Direct microcopy with 40% potassium hydroxide showing white homogenous grain; (E) Colonies of Gordonia soli in blood agar culture medium after 72 h of incubation at 30ºC.

FIGURE 2: Clinical features; (A) Nodular lesions on left foot compatible with mycetoma; (B) Recurrence of symptoms after several years of medical treatment; (C) Magnetic resonance imaging (MRI) of left foot, revealing high-signal areas on fat-saturated T2 weighted sequence in the subcutaneous tissue and midfoot bones. Red arrow indicates tiny hypointense foci within hyperintense spherical lesions, known as the “dot-in-circle” sign.